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Anti-Ubiquitin/UBC/UB抗體熱銷

時間:2014/4/10閱讀:271
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 Anti-Ubiquitin/UBC/UB抗體熱銷

FROM:ABCAM
Catalog Number : BYK-1549R       
Quantity size :   0.1ml  (dilute with pH 7.4    0.01 M PBS or antibody diluent )

Anti-Ubiquitin/UBC/UB抗體熱銷Background: Ubiquitin is a highly conserved protein, which has an ATP dependent role in the targeting of proteins for proteolytic degradation. To perform this function, the protein to be degraded is first covalently attached to the C terminus of ubiquitin, and the ubiquitinated complex is then recognized by a complex of degradative enzymes. Interestingly, ubiquitin also becomes covalently bonded to many types of pathological inclusions, which appear to be resistant to normal degradation. Therefore, ubiquitin antibodies are very useful for studies of these inclusions. For example, the neurofibrillary tangles and paired helical filaments diagnostic of Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease are all heavily ubiquitinated and can be readily visualized with ubiquitin antibodies.

Protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Attachment to proteins as a Lys-48-linked polymer usually leads to their degradation by proteasome. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous functions, including maintenance of chromatin structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair.

Anti-Ubiquitin/UBC/UB抗體熱銷Specificity :   

  • Anti -Ubiquitin/UBC/UB is a rabbit polyclonal antibody unconjugated                
  • specific for Ubiquitin/UBC/UB of mohu, rat         
  • use for western blotting,elisa,immunoprecipitation and immunohistochemistry
  • Protein G affinity chromatography purification, purity :>95%
  • Isotype:  IgG
  • mol wt:  8.5/17/34/75kDa

Application :  

  • Western blotting 1:100-500                
  • Immunohistochemistry 1:100-500
  • IF=1:100-500  IP=1:20-100                 
  • ELISA 1:500-1000
  •  IHC-P=1:100-500  IHC-F=1:100-500                  
  • Optimal working dilutions must  be determined by the end user.

Anti-Ubiquitin/UBC/UB抗體熱銷Storage:  Store at -20 for one year. Avoid repeated freeze/thaw cycles. The  lyophilized antibody is stable at room temperature for at least one month and for greater  than a year when kept at -20. When reconstituted in sterile pH 7.4 0.01M PBS or diluent  of antibody, the antibody is stable for at least six weeks at 2-4

Important Note: This product as supplied is intended for research use only, not for use in  human, therapeutic or diagnostic applications.

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