背景介紹：Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. The 28S subunit of the mammalian mitoribosome may play a crucial and characteristic role in translation initiation. This gene encodes a 28S subunit protein that is one of the more highly conserved mitochondrial ribosomal proteins among mammals, Drosophila and C. elegans. Splice variants that differ in the 5' UTR have been found for this gene; all variants encode the same protein. Pseudogenes corresponding to this gene are found on chromosomes 1q, 4p, 4q, and 20q 

別名：28S ribosomal protein S33; 28S ribosomal protein S33, mitochondrial; AI841153; CGI 139; FLJ21123; Ganglioside induced differentiation associated protein 3; Gdap3; MGC106490; mitochondrial; MRP S33; MRP-S33; MRPS33; OTTHUMP00000212181; OTTMUSP00000028636; OTTMUSP00000028637; PTD003; RT33_HUMAN; S33mt.   

28S核糖體蛋白S33抗體,Anti-RT33抗體簡單介紹：

產品編號：BYK-18882R

英文名稱：Anti-RT33
中文名稱：28S核糖體蛋白S33抗體

產品規格：50ul；100ul；200ul；1ml，*

抗體來源 Rabbit or Mouse

克隆類型 Polyclonal or Monoclonal

交叉反應、產品應用 （具體交叉反應與產品應用請或添加客服直接索取）

性    狀 Lyophilized or Liquid
濃    度 1mg/ml

28S核糖體蛋白S33抗體,Anti-RT33抗體產品信息：

博研生物小課堂：

常用激光器對應熒光素的選擇：

藍色激光器熒光素（488nm）：Alexa Fluor 488、FITC、PerCP、PerCP-Cy5.5、PE、PE-Cy5.5、PE-Cy5、PE-Cy7

紅色激光器熒光素（633nm）：APC、Cy5、APC-Cy5.5、APC-Cy7、Alexa Fluor 647

黃色激光器熒光素（561nm）：PE、PE-Cy5.5、PE-Cy5、PE-Cy7、Cy3、Alexa Fluor 555、Alexa Fluor 594

同型對照抗體的選擇

同型對照（Isotype Control），用于消除由于抗體非特異性結合到細胞表面而產生的背景染色，是真正意義上的流式陰性對照，流式細胞實驗*的一項。同型對照是使用與一抗相同種屬來源、相同亞型及亞鏈、相同熒光標記的免疫球蛋白，也要求使用相同劑量。

純化級別抗體：如果是純化的一抗加熒光標記的二抗，那么應該選擇與一抗相對應的同型對照抗體（如樣品管為：純化的CD3+PE標記的二抗+樣本；則對照管為：純化的同型對照+ PE標記的二抗+樣本）。

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